Structural consequences of dry heating on alpha-lactalbumin and beta-lactoglobulin at pH 6.5

Structural consequences of dry heating on alpha-lactalbumin and beta-lactoglobulin at pH 6.5

pH-induced structural transitions in beta-lactoglobulin.

Pharmaceutique units/mg; Novo, Copenhagen, Denmark) and a-chymotrypsin (Choay, Paris, France) were, under appropriate conditions, added to the partially purified enzyme in final concentrations of 50 and 250 pg/ml and incubated at 37°C for different time intervals, ranging from 1 to 24 h. In all proteinase-treated preparations the DPP IV activity measured exceeded 90% of the original DPP IV acti...

Transport of beta-lactoglobulin and alpha-lactalbumin in enterocyte-like Caco-2 cells.

The transport of [14C]-radiolabelled beta-lactoglobulin and alpha-lactalbumin through Caco-2 cell monolayers grown on permeable filters was studied in order to evaluate the different protein pathways through the intestinal epithelium. beta-Lactoglobulin or alpha-lactalbumin (0.25-3 mg/ml) was introduced on the apical side of the monolayer and both the transport and the release of labelled mater...

Differential Expression of Alpha S1 Casein and Beta-Lactoglobulin Genes at Different Physiological stages of the Adani Goats Mammary Glands

Background: Milk proteins genes have been the focus of the researches as the candidate target genes that play a decisive role when animal breeding is desired.Objectives: In the present study, the transcriptional levels of Beta-lactoglobulin (BLG) and Alpha S1 casein (CSN1S1) genes were investigated during prenatal, milking and drying times in mammary glands of the Adani goats which showed...

The unfolding of beta-lactoglobulin at pH 3 by urea, formamide, and other organic substances.

We have reported in a previous paper (1) that optical rotatory dispersion measurements indicate that the native conformation of P-lactoglobulin, in aqueous solution, contains few, if any, regions with an a-helical structure. It appears likely, from the many studies of Jirgensons (2) that this conclusion is applicable to a large number of globular proteins, and we have suggested that this indica...